RPMI Medium 1640 細胞培養(yǎng) solarbio

RPMI Medium 1640，含L-谷氨酰胺、酚紅，不含丙酮酸鈉、HEPES

紅細胞裂解液的配置方法

取100 μl抗凝全血，加2 ml應(yīng)用液3#，混勻15～20 s；加2 ml應(yīng)用液2#，混勻15～20 s；加4 ml應(yīng)用液1#，混勻15～20 s；300×g離心2 min，棄上清；收集沉淀，加PBS緩沖液，制成白細胞懸液，備用。（這種紅細胞裂解法滿足了白細胞流式細胞儀檢測分選要求，價格低廉，是一種簡便快捷、經(jīng)濟實用的白細胞純化分選方法。）

紅細胞裂解液的注意事項

1、本裂解液為無菌產(chǎn)品，分離細胞用于細胞培養(yǎng)時請注意無菌操作。

2、為了您的安全與健康，請穿實驗服并戴一次性手套操作。

3、 儲存液儲存于2-8°C不能超過半年(六個月)。

4、使用時根據(jù)需要稀釋,每次用都應(yīng)用去離子水新鮮配制工作液,當(dāng)日用不完的可廢棄。

北京索萊寶科技有限公司為紅細胞裂解液生產(chǎn)廠家，貨號為R1010，常賣規(guī)格為100ml 、500ml。索萊寶生產(chǎn)的紅細胞裂解液是利用細胞內(nèi)外存在鹽離子濃度差而導(dǎo)致細胞膜脹破的原理來裂解無核紅細胞的。產(chǎn)品經(jīng)過無菌處理，主要用于經(jīng)酶消化分散的組織細胞的分離純化，淋巴細胞的分離純化以及組織細胞蛋白與核酸提取等實驗中紅細胞的去除。

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RPMI Medium 1640 細胞培養(yǎng) solarbio